Package: pcr 1.2.2
pcr: Analyzing Real-Time Quantitative PCR Data
Calculates the amplification efficiency and curves from real-time quantitative PCR (Polymerase Chain Reaction) data. Estimates the relative expression from PCR data using the double delta CT and the standard curve methods Livak & Schmittgen (2001) <doi:10.1006/meth.2001.1262>. Tests for statistical significance using two-group tests and linear regression Yuan et al. (2006) <doi:10.1186/1471-2105-7-85>.
Authors:
pcr_1.2.2.tar.gz
pcr_1.2.2.zip(r-4.5)pcr_1.2.2.zip(r-4.4)pcr_1.2.2.zip(r-4.3)
pcr_1.2.2.tgz(r-4.4-any)pcr_1.2.2.tgz(r-4.3-any)
pcr_1.2.2.tar.gz(r-4.5-noble)pcr_1.2.2.tar.gz(r-4.4-noble)
pcr_1.2.2.tgz(r-4.4-emscripten)pcr_1.2.2.tgz(r-4.3-emscripten)
pcr.pdf |pcr.html✨
pcr/json (API)
NEWS
| # Install 'pcr' in R: |
| install.packages('pcr', repos = c('https://mahshaaban.r-universe.dev', 'https://cloud.r-project.org')) |
Bug tracker:https://github.com/mahshaaban/pcr/issues
data-analysesmolecular-biologyqpcr
Last updated 4 months agofrom:0c5242ce9b. Checks:OK: 1 NOTE: 6. Indexed: yes.
| Target | Result | Date |
|---|---|---|
| Doc / Vignettes | OK | Nov 12 2024 |
| R-4.5-win | NOTE | Nov 12 2024 |
| R-4.5-linux | NOTE | Nov 12 2024 |
| R-4.4-win | NOTE | Nov 12 2024 |
| R-4.4-mac | NOTE | Nov 12 2024 |
| R-4.3-win | NOTE | Nov 12 2024 |
| R-4.3-mac | NOTE | Nov 12 2024 |
Exports:pcr_analyzepcr_assesspcr_curvepcr_dctpcr_ddctpcr_efficiencypcr_lmpcr_standardpcr_testpcr_ttestpcr_wilcox
Dependencies:clicolorspacefansifarverggplot2gluegtableisobandlabelinglatticelifecyclemagrittrMASSMatrixmgcvmunsellnlmepillarpkgconfigR6RColorBrewerrlangscalestibbleutf8vctrsviridisLitewithr
Readme and manuals
Help Manual
| Help page | Topics |
|---|---|
| C_T values from qPCR (separate tubes) | ct1 |
| C_T values from qPCR (same tubes) | ct2 |
| C_T values from qPCR (Serial dilutions) | ct3 |
| C_T values from qPCR (Serial dilutions) | ct4 |
| 'pcr' package | pcr |
| Apply qPCR analysis methods | pcr_analyze |
| Assess qPCR data quality | pcr_assess |
| Calculate the standard curve model | pcr_curve |
| Calculate the delta_ct model | pcr_dct |
| Calculate the delta_delta_ct model | pcr_ddct |
| Calculate amplification efficiency | pcr_efficiency |
| Linear regression qPCR data | pcr_lm |
| Calculate the standard curve | pcr_standard |
| Statistical testing of PCR data | pcr_test |
| t-test qPCR data | pcr_ttest |
| Wilcoxon test qPCR data | pcr_wilcox |
